Investigating The Effect Of Kajwa Herbal Product On Possible Effects On Liver And Osteoporosis Caused By Glucocorticoid In Rats

Abstract

Osteoporosis is a disease with decreased bone density and loss of bone microstructure, which in turn increases the risk of various fractures. This disease is one of the most important causes of disability in the elderly, especially women. One of the important complications of osteoporosis is pathological fracture, which in some cases can be fatal. Nowadays, various prevention and treatment methods, including pharmacological and non-pharmacological methods (such as alendronate and parathyroid hormone) are available against this disease. Given the chemical nature of the drug compounds and the side effects of current treatments, it seems appropriate to find a herbal alternative. This study was designed and performed to determine the prophylactic effect of Kajwa plant product on glucocorticoid-induced osteoporosis in rats.

Methods: In this study, 35 male Wistar rats weighing in the range of 250-200 g were used in the groups including control, Normal saline 0.9% (negative control) + 0.1 mg / kg dexamethasone by subcutaneous injection, Kajwa herbal composition with a dose of 500 mg / kg + 0.1 mg / kg dexamethasone as a subcutaneous injection, Kajwa herbal composition with a dose of 1000mg / kg + 0.1 mg / kg dexamethasone as a subcutaneous injection, Alendronate (1 mg / kg / day) + 0.1 mg / kg dexamethasone by subcutaneous injection for 2 months.

Serum levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) per liter (U / L) and lipid profile including measurements of serum levels of total cholesterol, HDL (High Density Lipoprotein), LDL (Low Density Lipoprotein) and triglycerides (mg / dL) were measured according to the instructions in each kit. Data were analyzed by SPSS software (19) using descriptive statistical tests (frequency percentage and mean and standard deviation) and ANOVA test in case of abnormal data distribution from its parametric bread equivalent (Kruskal Wallis).

Findings: Comparison of the mean levels of liver enzymes in the studied rats showed that there was no significant difference between the different groups (P> 0.05). The results of serum cholesterol, triglyceride and HDL levels did not show a significant difference between different groups of rats. The results of this study also showed that there was a significant difference in serum creatinine levels between the different groups, so that the group receiving dexamethasone was significantly higher than the other groups (P <0.05). Co-administration of kajwa with dexamethasone improved dose-dependent histology of bone tissue.

Conclusion: Kajwa can have beneficial effects in improving dexamethasone-induced osteoporosis. Also, doses of 500 and 1000 mg / kg of this compound do not have a detrimental effect on the biochemical factors under study.