Development And Validation Of UV-Visible Spectrophotometric Method For Estimation Of Chrysin In Bulk Form

Abstract

The goal of this work is to establish a straight forward UV-visible Spectrophotometric approach for determining chrysin in its pure form and to validate the method that has been devised. Using a UV-visible double beam spectrophotometer and a solvent solution containing dimethyl sulfoxide, a method was developed and validated for chysin. By following the International Conference on Harmonization (ICH) recommendations, analytical properties like linearity and precision were determined. According to Beer's law, the devised method demonstrated linear response for chrysin concentrations between 1 and 5 μg/ml, and a coefficient of correlation of 0.9987 was discovered. The method's sensitivity was determined and the limits of detection (LOD) and limit of quantification (LOQ) were discovered to be 0.2155 μg/ml and 0.6531 μg/ml respectively. The study found that the method for estimating chrysin is linear, consistent, precise and affordable and can be used to analyse pharmaceutical formulations.