Effect of Supplemented Diet with Ginkgo Biloba Leaves Powder on Some Biochemical Parameters and Minerals Content in Cisplatin-Induced Nephrotoxicity in Rats

Abstract

Nephrotoxicity is one of the most common kidney problems which occurs when the body is exposed to toxic drugs or toxins. The present work aimed to investigate the effect of Ginkgo biloba leaves powder (GBLP) on some biochemical parameters and minerals content in Cisplatin (Cis)-induced nephrotoxic rat model. Forty adult male Sprague-Dawley rats (body weight 170±10 g) were randomly divided into eight groups, of 5 rats each. Group 1 negative control: was fed on the basal diet only. Groups 2, 3 and 4 were fed on basal diet mixed with GBLP at 1, 2 and 4% concentrations, respectively. Group 5: positive control: was intraperitoneally injected with a single dose of Cis at 7 mg/kg b. wt. to induced nephrotoxicity in rats. Groups 6, 7 and 8 were fed on basal diet mixed with GBLP at 1, 2 and 4% levels, respectively and injected with Cis as mentioned before. At the end of the experimental period (6 weeks), the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), blood urea, creatinine, total proteins, albumin, malondialdehyde (MDA), glutathione (GSH) and minerals contents were determined. In addition, the effect of feeding GBLP on formalin-induced local inflammation in the paw's thickness was measured at different periods.  Diet supplementation with GBLP improved the above mention biochemical parametrs at 2 and 4%. Feeding on GBLP prior to Cis injection produced protective effects and returned the biochemical parameters to nearly normal levels. The protective effect of GBLP was more pronounced at the high level (4%). In conclusion, the beneficial effects of GBLP may be ascribed to its hepatoprotective, renoprotective, antioxidant and anti-inflammatory properties and high levels of minerals content.