Lack of hypoglycemic activity in total flavonoid fraction of Wrightia tinctoria on alloxan induced hyperglycemia

Abstract

Objective: The objective of this study was to investigate the hypoglycemic activity of
Wrightia tinctoria (Roxb) (R. Br. Linn., Family‑ Apocynaceae) total flavonoid (WTTF)
fraction on alloxan induced hyperglycemia. Materials and Methods: Total flavonoid
fraction from WTTF seed was separated and LD50 calculated as 1006.93 mg/kg, i.p.,
following Organization for Economic Co‑operation and Development guideline 423.
The effect of total flavonoid fraction was observed in three different dose levels 50,
75, and 100 mg/kg. Hypoglycemic activity was carried out using alloxan induced
hyperglycemia model. The parameters observed were body weight, blood glucose, total
cholesterol, triglycerides(TG), low density lipoprotein, high density lipoprotein, urea and
creatinine along with relative organ weight of liver, kidney, and pancreas. Results: Total
flavonoid fraction of W. tinctoria did not have any significant effect on reducing glucose
level. WTTF had significantly reduced creatinine (P < 0.001), TG (P < 0.05) level
and relative weight of liver in diabetic rat at the dose of 100 mg/kg. Conclusion: All
the findings indicate absence of antidiabetic activity in W. tinctoria seed but possible
presence of liver and kidney protective property