BIOACTIVATED MARINE COLLAGEN MATRIX WITH INJECTABLE PLATELET RICH FIBRIN IN SOFT TISSUE AUGMENTATION - AN IN-VITRO WOUND HEALING STUDY

Abstract

Objectives: The study intended to assess the efficacy of collagen matrix of marine origin impregnated with injectable plasma rich fibrin (I-PRF) on fibroblast migration and viability in comparison with plain collagen matrix.

Methodology: Standard I-PRF obtained by centrifugation of 10ml of whole blood at 700 rpm (60G force) for 3 minutes was impregnated in collagen scaffold, was checked for fibroblast biocompatibility at 24 hours (MTT assay & fluorescent microscopy); for fibroblast migration at 24, 48, 72 hours, and results were compared with plain collagen matrix.

Result: Cell viability assay showed the viability of 98.8% for collagen and 98.1% for collagen with I-PRF. Cell migration at 24, 48 and 72 hours were 4.2% and 12.5%, 47.9% and 50%, 77% and 98.75% for collagen and collagen with I-PRF respectively.

Cell viability assay showed no cytotoxicity with collagen and collagen impregnated with I-PRF and cell migration was found to be better in collagen impregnated with I-PRF.

Conclusion: Marine Collagen and marine Collagen with I-PRF showed the potential for tissue regeneration via induction of fibroblast behavior. Bioactivation of collagen matrix with I-PRF might enhance soft tissue healing and tissue regeneration.