SCREENING OF ANTIBIOTIC RESISTANCE GENES IN VIBRIO AND AEROMONAS SP. ISOLATED FROM THAI RICE-FERMENTED FISH PRODUCTS

Abstract

Vibrio and Aeromonas sp. are genus of foodborne pathogens especially occurred in aquatic animals and environments. is also commonly isolates from aquatic organisms and environments, which can cause various symptoms in human, commonly gastro-intestinal diseases. Antibiotic resistance properties of Vibrio and Aeromonas sp. pathogens against multiple antibiotic drugs had reported. Thai traditional rice-fermented fish products including, pla-som (n = 15) and pla-chom (n = 15) are risk to contaminate with pathogenic bacteria. The study was aimed to identified Vibrio and Aeromonas sp. isolated from pla-som and pla-chom; and to evaluate antibiotic resistant properties and screen antibiotic resistant genes of isolated bacteria. According from colony, Gram s staining and biochemical characteristics, Vibrio and Aeromonas sp. were identified. Antibiotic susceptibility of identified bacteria were determined against fifteen antibiotic discs. Screening of antibiotic resistance genes of Vibrio and Aeromonas sp. were also evaluated. All of isolated bacteria were possessed antibiotic resistance properties and multiple antibiotic resistances, which were occurred in V. vulnificus (n = 3), V. mimicus (n = 1), V. harveyi (n = 3) and A. hydrophila (n =3). Mostly of antibiotic resistance genes including vhhP2, tl and rpoS genes, were reserved in V. vulnificus isolates. V. parahaemolyticus and V. harveyi were carried tl and rpoS genes; and vhhP2 gene, respectively. Therefore, no antibiotic resistance genes were determined from V. mimicus, V. cholerae and A. hydrophila isolates. V. vulnificus isolated from this product was represented the relationship between phenotypic and genotypic occurrences, and ampicillin, chloramphenicol and cefuroxime were common antibiotic resisted.