PHYTOCHEMICAL SCREENING AND BIOLOGICAL ACTIVITY OF FINGER-ROOT (BOESENBERGIA ROTUNDA L.) RHIZOME ON SKINCARE APPLICATION

Abstract

Traditional plants are most attractive ingredient of skincare products. The research purposes were to screen phytochemical constituents contained in ethanol extract of fingerroot (Boesenbergia rotunda L.) rhizome; and to evaluate in vitro biological activities on skincare, including antioxidant, anti-inflammation, anti-melasma, anti-wrinkle and its cytotoxicity against human skin fibroblast cells. Ethanol extract of fingerroot rhizome (FRE) was positive for triterpenes determined by qualitative phytochemical screening. Total phenolic content (TPC) and total flavonoid content (TFC) were 91.51±0.76 milligram of gallic acid equivalent per gram and 21.05±1.53 milligram of quercetin equivalent per gram, respectively. FRE was scavenged only DPPH radicals (IC50 = 3.80 ±0.04 mg/ml). FRE (1 mg) was demonstrated in vitro anti-inflammation property by reduced nitric oxide (NO) production (24.5±4.07%) from LPS-induced macrophage cells. FRE was also inhibited tyrosinase (IC50 = 0.11 ±0.03 mg/ml) and can compared with kojic acid. Therefore, FRE was lack of anti-elastase activity. FRE (0.0001-1.0 mg/ml) was non cytotoxic against human skin fibroblasts. In this study, we were reported in vitro anti-melasma activity of FRE as anti-tyrosinase inhibitor, therefore it was lack of anti-wrinkle activity due to unable to inhibit elastase enzyme. The finding was supporting that FRE can be ingredient of skincare products as antioxidant, anti-inflammation, anti-tyrosinase inhibitor and skin disorder relive without harmful effect.