Evaluation Of Wound Healing And Anti- Inflammatory Effect Of Topical Formulations Of Boswellia Serrata Using Adult Zebra Fish And Macrophage Raw 264.7 Cells

Abstract

A large majority of the population in the world have employed medicinal plants for their therapeutic needs. There is an urgent need to
find scientific evidence of the therapeutic nature of medicinal plants to be used in general practice. Inflammation is a common risk
factor in the pathogenesis of conditions such as infections, arthritis, type 2 diabetes mellitus, obesity and cancer. The aim of the present
study is to investigate the anti-inflammatory effect of the Boswellia serrata by formulating topical agents of gel and ointment using
adult zebra fish model and macrophage RAW 264.7 cells The study has employed the utilization of adult zebrafish to evaluate the
wound healing efficiency of the ointment and gel formulation of the B.serrata extract. In vitro anti-inflammatory activity was conducted
by albumin denaturation assay, anti-proteinase activity, and membrane stabilization assay which showed a significant % inhibition
when compared with standard Aspirin. In this study, we investigated the anti-inflammatory activities of B.serrata extract in
lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. It was seen that the ethanolic extracts of B.serrata significantly repressed
the production of inflammatory mediators such as nitric oxide and the expression of pro-inflammatory cytokines in the LPS-stimulated
RAW 264.7 cells. Caudal fin transection model was employed for induction of inflammatory wound healing using zebrafish. The
parameters for evaluation of wound healing efficacy is the measurement of regenerative capacity of the fin and neutrophil estimation.
Histopathological examination of wound was also performed. Upon treatment of 500 μg of ointment, the fishes shows a maximum
amount of fin regeneration by comparison with gel preparation. Utilisation of the 500 μg of ointment also revealed a high amount of
neutrophil count at the side 24 hours after the infliction of wound and a reduction in the inflammatory mediators after one day.