Gene expression study of Pvl genes in Methicillin- Resistant Staphylococcus aureus bacteria on the effect of extract Tribulus terrestris L.

Abstract

125 samples were collected from different clinical sources including {burns, wounds, ear, suppurations and boils, urine} from Ramadi Teaching Hospital and Women’s and Children’s Hospital in Ramadi for the period from {11-2021 to 1-2022}. 50 isolates of Staphylococcus aureus were found, of which 26 were MRSA isolates, with an isolate rate of {52%}. The ethanolic extract of the fruits of the Tribulus terrestris Lplant was prepared and its effectiveness against the isolates was tested by diffusion around the pits. The ethanolic extract had anti-MRSA activity by8 5%. The general average diameter of the inhibition for isolates was 18.038 mm, and the general average for MIC was 14.76 mg/ml. The results of GC-MS showed that the extract contained 49 effective chemical compounds, the most important of which are phytol, pyrrole, saturated and unsaturated fatty acids, alkaloids, saponins, tannins and phenols. The gene expression results showed that the MRSA bacteria treated with sub-MIC for the plant extract reduced the gene expression of PVL genes {lukS-pv and lukF-pv} to 0.506,0.716 respectively compared to the gene expression {1} of the control group {untreated bacteria}. It was found that the ethanolic extract of the plant is able to degrade red blood cells at a concentration of 750 mg/ml