Evaluation Of Antiurolithiatic Activity Of Methanolic Seed Extracts Of Persea Americana Against Calcium Oxalate Induced Urolithiasis In Rats

Abstract

Objective: Persea Americana methanolic seed extracts are considered for the present study to access its efficacy, urolithiasis is induced
by administering the male rats with calcium oxalate.
Methods: Calcium oxalate urolithiasis is induced in male rats by means of hyperoxaluria model by administering ethylene glycol of
0.75% v/v, with 1% w/v ammonium chloride supplemented in drinking water for 3 days followed by 0.75% v/v of ethylene glycol for
25 days. The PAMSE is administered to urolithiasis induced test group at dose of 150mg/kg respectively for 28 days.
Results: As anticipated after 28 days the calcium oxalate is deposited significantly in the kidney which is associated with the increase
of Urinary oxalate, Urine calcium & magnesium levels, serum uric acid, creatinine in urolithiasis control groups. ACP, ALP, AST &
ALT well known biochemical parameters are seen to be increased with decreased level of LDH assessed using kidney homogenate,
copiously confirming the induction of urolithiasis , its promising to know that the PAMSE intubation to the test group daily decreased
the quantity of formation of calcium oxalate and all the biochemical changes induced by urolithiasis is reverted.
Conclusion: Results from this study shows that PAMSE is effective inhibitor of crystallization of calcium oxalate comparable to the
standard Cystone in dose dependent mode. Therefore indicating PAMSE’s significant antiurolithiatic activity.