Comparative Analysis Of Chemical Constituent And Antimicrobial Potential Of Acorus Calamus Linn (Sweet Flag) In The Different Geographical Regions Of North Himalayas

Abstract

Sweet flag (Acorus calamus) is a well-known plant all over the world due to its specific bioactive components β- asarone in plants.
The present study reports the quantitative estimation of rhizome oil of the sweet flag plant; obtain from the various district of
Uttarakhand, and different geographical surroundings. GC-MS is used to estimate the quantity of rhizome oil. Although leaves are
enriched by the several medicinal constituents revealed by the previous studies, rhizome, and the oil it is being investigated. The main
component which was identified was Cis-ocimene, Linalool, Trans methyl isoeugenol, β and α-asarone, and euasarone. β-asarone in
the different samples; showed a higher percentage as compared to another component. It was observed that the Gopeshwar region
sample had the 74.36 least percentage and the Pauri sample had the 92.22 highest percentage of β-Asarone. Meanwhile, the Gopeshwar
region sample had 17.25 highest percentage, and the Pauri sample had 1.57 least percentage of α-Asarone. Fungal infection is prevalent
nowadays. The oil from all regions subjected to antifungal activity against the Aspirigilla fumigates, A. Parasiticus, Microsporum
Canis, M. gypsum, Cladosporium, Aspergillus niger. The inhibition zone diameter (IZD) against Aspirigilla fumigates, A. parasiticus
and was a maximum of 10cm for the Gopeshwar sample,9cm for the Ramnagar sample as compared to the reference drug gentamicin.