UNVEILING THE APOPTOTIC POTENTIAL OF SILVER NANOPARTICLES ON HUMAN COLON CARCINOMA HT29 CELLS
DOI:
https://doi.org/10.47750/pnr.2022.13.S06.338Keywords:
Silver nanoparticle, Cytotoxicity, HT29 cells, Apoptosis, AO/EtBr .Abstract
The synthesis of nanoparticles is an essential element of nanotechnology, because of its potential for use in chemotherapies, the interest in
this area of research is expanding. For the synthesis of silver nanoparticles, the chemical reduction technique was used. According to these
investigations, the particles have an average size of 16 nm and are mainly spherical in shape. Additionally, A dose-dependent cytotoxicity
was also shown by the produced AgNPs against a human colon cancer cell line (HT29). At 72 hours of incubation, it was discovered that
the inhibitory concentration (IC50) values for HT29 cells were 48.12 g/ml. The use of AO/EtBr labelling demonstrated an apoptotic
induction. The current findings clearly suggested that HT29 cell lines treated with AgNPs would exhibit anticancer activity.
