Biochemical And Heamatological Evaluation Pattern Of Pentostam- Induced Hepatotoxicity And Nephrotoxicity In Albino Mice

Abstract

This study was designed to examine the effect of different dose of Pentostam administration in male albino mice as a model, to
determine their effect on some biochemical and heamatological parameters. 28 adult male of BALB/c wild mice aged 8- 12 weeks and
weighing 25-39 gm were used and i.p. injected with 10mg/kg, 20mg/kg, and 40mg/kg pentostam in addition to a control group. After
28 therapeutic days, blood sample were collected from each animal. The result of Biochemical Index of the serum obtained showed
that there were increase significant differences between different concentration doses on AST, ALT, ALP, Urea, Creatinine and BUN
as compare to control group at (p >0.01). Conversely in case of Albumin and Total protein were the result showed decrease significant
difference between different concentration doses on this parameter as compare to control group of the same at (p> 0.01). while no
significant differences between the different concentrations group of doses on Total bilirubin, where the F value was (1.00). The result
of Hematological Index of blood sample showed that there were decrease significant difference at (P ≤ 0.01-0.05) in hemoglobin, HCT,
WBCs, LYM and MON concentration in the different concentrations dose of treatment as compared to control group. Conversely, for
MCHC, NEU and EOS there were increase significant difference at (P ≤ 0.05-0.01) as compared to the control group. While no
significant changes (P ≤ 0.0-01.05) in the parameters of RBCs, MCV, MCH, BAS count and Platelet were found when compared with
control animals. Biochemical and hematological evaluation pattern still an important role to evaluate drug toxicity. However, the
results of this study concluded that, different doses of Pentostam can cause hepatotoxicity and nephrotoxicity. Therefore, it will be
important of change in anti-leishmanial regimen to reduce their side effect