Cytotoxicity Assessment for Root Canal Sealers

Abstract

The purpose of this experimental study was to evaluate the cytotoxicity of four root canals sealer at different times Extract of set root canal sealer (watch’s, Endomethazon, refill, and Ditket)were added to cultured vero cells . The cytotoxic effect due to these extracts was evaluated after 24h,48h, and 72h by the Elisa reader which measures the optical density of each well at the end of the experimental time. The result showed that all sealers were cytotoxic. Watch’s sealer was significantly less toxic than other sealers on 1st and 2nd experimental days The highest cytotoxic effect appeared with Endomethazon then Dorifill and Diket On the 3rd experimental day no significant cytotoxic effects were found between all saler and statistically all sealer had lost 36-45 percent of there cytotoxicity