Effect of Ricinus Communis Aqueous Leaf Extract on the Healing of Wound. An in Vitro Study in the Cell Line

Abstract

Background: Wound is characterized as harm or disturbance to the typical anatomical organization and function. As a result, muscles,
nerves, arteries, tendons, parenchymal organs, and even bone may be harmed. It can also cause a basic breach in the tissue's epithelial integrity, such as dermal tissue, alternatively it may extend farther deeply into the subcutaneous tissue. Ricinus communis is a plant belong to the vast and significant family of flowering plants known as the Euphorbiaceae, different parts of the plant have bioactivity against a variety of diseases, include diabetes, wound infections, constipation, paralysis, and discomfort. There are a variety of phyto-constituents present, which contributes to these medicinal applications, includes reducing sugars, poly-uronides, glycosides, terpenoids, flavonoids, alkaloids, tannins, saponins, and steroids.
Aim of the study: To look into the impact of Ricinus Communis aqueous leaf extract on wound healing in MDCK cell line.
Materials) and (Methods: vertical wound was made by using 200μl sterile plastic micropipette tip to press firmly against the cell monolayer of tissue culture plate. Then the wounded MDCK cell was exposed to different concentrations of Ricinus communis aqueous leaf extract (500, 250, 125, 62.5, 31.25, 15.6) µg/ml.
Results: A) the concentration 125 µg/ml of R. communis aqueous extract caused significant increase in the cell viability percentage (p≤0.05) of the MDCK normal Cell line B) all concentrations of Ricinus communis aqueous leaf extract caused highly significant decrease (p<0.001) in the diameter of the MDCK normal cell line induced wound after 3,6,9 hours of incubation and wound healing was completed after 12 hours of incubation.
Conclusion: On the MDCK cell line, all concentrations of R.communis leaf aqueous extract have wound healing effect