Influence of Indole-3 butyric acid and 6- benzylaminopurine with Sucrose on in vitro Potato Microtuber Formation

Abstract

The research aim was to study the influence of sucrose with a combination of growth regulators: Indole-3-butyric acid (IBA) and 6-benzylaminopurine (BAP) on potato in vitro micro tuber formation. Under two levels of sucrose, three potato cultivars, “Nevsky”, “Riviera” and “Zefira” were studied for in vitro tuberization response: Murashige and Skoog (MS) media supplemented with 80 g.L−1
sucrose (8% MS medium) and 100 g.L−1 (10% MS medium). Two concentration (0.05 mg.L−1 and 0.1 mg·L−1) of growth regulators: indole-3-butyric acid (IBA) and 6-benzylaminopurine (BAP) were tested in this work. Potato varieties "Zefira", "Nevsky" reacted positively on high concentration of growth regulators (0.1 mg.L−1 BAP + 0.1 mg.L−1IBA) on in vitro tuberization process, while the concentration of IBA increased from 0.05 mg.L−1 to 0.1 mg.L−1 potato variety "Riviera" had a negative results on the microtuber germination rate. Finally, the optimal concentration of sucrose (10 g.L−1) and growth regulators (0.1 mg.L−1 BAP + 0.1 mg.L−1 IBA) for tuberization of potato varieties "Zefira" and "Nevsky" has been selected. The best protocol (8% MS medium +0.1 mg.L−1 BAP + 0.05 mg.L−1 IBA) for in vitro tuber induction of varieties ” Riviera” was developed .