Absence of anti‑Staphylococcus aureus (MRSA) activity of secondary metabolite actinomycetes associated sponges from Pulau Panjang, Indonesia

Authors

  • Yatnita Parama Cita

Keywords:

Anti‑methicillin resistant Staphylococcus aureus, Pulau Panjang, secondary metabolite actinomycetes‑associated sponges

Abstract

Objective: To study the anti‑Staphylococcus aureus MRSA of the secondary metabolite
actinomycetes associated sponges from Pulau Panjang, Central Java, Indonesia.
Materials and Methods: The sponge samples were taken by scuba diving in Pulau
Panjang, and the sponge was identified using external morphology as well as identification
of spicules from the fresh preparation. Isolation of actinomycetes associated with sponge
was conducted by means of direct plating. The symbion bacteria were planted (scratched
in a circular way) on agar medium Zobell 2216E, medium actinomycetes using the
existing method, namely, S.aureus MRSA and E. coli MRSA. Results: The results of
identification from 6 sponges were Phakettia euctimenna, Gelliodes fibolatus, Lamellodysidea
herbacea, Clathria reindwardtii and Callyspongia sp, while antibacterial assay for the
secondary metabolite actinomycetes associated sponges showed negative results.
Conclusion: Secondary metabolite actinomycetes associated sponges from Pulau
Panjang were not found to have antibacterial activity against S. aureus MRSA.

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Published

2015-01-28

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Articles

How to Cite

Absence of anti‑Staphylococcus aureus (MRSA) activity of secondary metabolite actinomycetes associated sponges from Pulau Panjang, Indonesia. (2015). Journal of Pharmaceutical Negative Results, 6(1), 33-36. https://pnrjournal.com/index.php/home/article/view/88