The Investigation of Methoprim in High Concentration Dose on Rats

Authors

  • Faisal.H.G.Q. Al-Amier
  • Zahraa Hatim Jabbar
  • Karam.R. Aljarah
  • Ghanem Shaker Al-Jebouri
  • Sarmad Al-Sahaf

Keywords:

Artemisia vulgaris, Cisplatin, Hepatoprotective, hepatotoxicity, herbs.

Abstract

Trimethoprim is a dihydropyrimidine antibiotic, antiparasitic, and antiparasitic agent Rash and pruritus are the side effects of trimethoprim that are most frequently reported. The digestive and haematopoietic systems were also engaged in other negative consequences that were described(5). After taking 1000 mg or more of trimethoprim, symptoms of acute overdose can include nausea, vomiting, headaches, dizziness, disorientation, mental depression, and bone marrow depression. 20 Healthy mature rats were used in this experiment. Their age was in the range between 3-5 months , and their weight was around 180±15g. They were divided randomly into four groups and five rats were kept in each cage. A significant increase in the AST enzyme level in the treated groups with 50,100 and 150 mg\kg b.wt. Respectively after 60 days of treatment compared to the control group. Also showed a significant increase in the ALT enzyme in the treated group with 50,100 and 150 mg\kg b.wt. after the passage of 60 days compared to the enzyme level for the control group in Table 1 revealed that there was significant (P≤0.05) increased in the ALP enzyme level in the treated groups with 100 and 150 mg\kg b.wt .(155.34 ±3.16and 188.31 ±4.91)IU\L respectively (80.33 ±2.03)IU\L compared with control group (85.43 ±3.32)IU\L after 60 days , The significant(P≤0.01). increase in blood urea level was found in the treated group with 100 and 150 mg\kg of b. wt.( 36.38 ±1.51 and43.42 ±0.35 ) mg/dl after 60 days compared with the control and treated groups with 50 mg\kg of b. wt.( 25.24 ±0.28and26.60 ±0.03 ) mg/dl.In the table NO.1, the results were showed significant(P≤0.01). increase in Creatinine concentration in the treated group with 150 mg\kg of b. wt.( 1.34 ±0.09) mg/dl days compared with the other three groups The results of the determination of malondialdehyde showed a significant (P≤0.05) increased in its concentration of MDA in the treated group with50, 100and150 mg\kg b.wt. (2.90 ±0.34, 4.34 ±0.23and5.22 ±1.24) nmol\lrespectively for a period of 60 days compared with the control (1.19 ±0.28). The results showed a significant (P≤0.01) increased in the level of catalase enzyme in the group treated with concentration 150 mg\kg b.wt.( 5.22 ±1.2 4) IU\L duration of 60 days compared with the control group(2.12±1.07) IU\L. while three was no significant difference in the level of catalase in treated group with 50 and 100 mg\kg b.wt.( 3.21 ±0.42 and 3.09 ±0.35) compared with control group. On the other hand, the results showed a significant increase in the level of the SOD enzyme in the two treated groups 100 and 150 mg\kg b.wt (4.03 ±0.93 and 4.43 ±0.62 ) IU\L for a period of 60 days compared to the control group(1.23 ±0.09)IU\L .The results did not show a significant difference in the level of SOD enzyme in the treated group with 50 mg\kg (2.89 ±0.26) IU\L as compared with control group (1.23 ±0.09 ) IU\L .

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Published

2022-09-17

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Section

Articles

How to Cite

The Investigation of Methoprim in High Concentration Dose on Rats. (2022). Journal of Pharmaceutical Negative Results, 849-859. https://pnrjournal.com/index.php/home/article/view/850