Insignificant antitubercular activity of pyrazoline, phenyl pyrazoline and isoxazoline moiety in lupeol

Authors

  • Vandana Khattar

Keywords:

Crataeva nurvala, lupeol aldehyde, Microplate Alamar Blue Assay, Mycobacterium tuberculosis, semisynthetic

Abstract

Aim: The Present study is to investigate the antimycobacterial activity of pyrazoline,
phenyl pyrazoline and isooxazoline moiety containing lupeol. The main purpose to
show insignificant antimicrobial activity of these lupeol derivatives and hence that
further researchers do not waste time to investigate antitubercular activity of these
group containing lupeol derivatives. Materials and Methods: Lupeol has been
isolated from Crataeva nurvala and its reported antitubercular activity prompted
to prepare semisynthetic derivatives to know how much they show antitubercular
activity. Newer substituted derivatives of lupeol were synthesized by firstly oxidation
of lupeol to lupeol aldehyde followed by replacement of aldehydic group by various
groups as pyrazoline, phenyl pyrazoline and Isooxazoline. These Pyrozoline, Phenyl
pyrazoline and Isooxazoline derivatives are synthetic analogues of the naturally occurring
triterpenoid lupeol from the plant crataeva nurvala, were tested for their spectral data
and conformation of the groups, then tested these derivatives of lupeol for antitubercular
activity against Mycobacterium tuberculosis (MTB) strain H37Rv using the Microplate
Alamar Blue Assay. Result: The result indicate that pyrazoline, Phenyl pyrazoline and
Isooxazoline moiety containing lupeol derivatives did not show antimycobacterial
activity against MTB at 50 µgm/ml. Conclusion: Pyrazoline, Phenyl pyrazoline and
Isooxazoline derivative of lupeol did not have significant antimycobacterial activity.
Thus, further optimization of it is needed.

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Published

2015-01-28

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Section

Articles

How to Cite

Insignificant antitubercular activity of pyrazoline, phenyl pyrazoline and isoxazoline moiety in lupeol. (2015). Journal of Pharmaceutical Negative Results, 6(1), 11-19. https://pnrjournal.com/index.php/home/article/view/85