Lack of in vitro anticancer and antimicrobial activities in Suaeda maritima (seablite) crude extracts

Abstract

Aims: The aim was to determine antimicrobial and anticancer activities for water and ethanol
extracts of leaves and shooting parts of seablite. Materials and Methods: Anticancer
activity tests of seablite extracts were performed by resazurin microplate assay (REMA)
and tested with 3 cell lines including KB cell line, epidermoid carcinoma of the oral
cavity (American Type Culture Collection [ATCC] CCL‑17), MCF‑7 cell line,
breast adenocarcinoma (ATCC HTB‑22) and NCI‑H187 cell line, and small cell lung
carcinoma (ATCC CRL‑5804). Antimicrobial activities of seablite water and ethanol
extracts against herpes simplex virus type‑I (HSV‑1) and Mycobacterium tuberculosis
H37Ra strain were tested according by green fluorescent protein‑based assay; Candida
albicans (ATCC 90028) and Bacillus cereus (ATCC 11778 or TISTR 687) were tested
according by REMA; and Plasmodium falciparum, K1 strain was tested according by
micro‑culture radioisotope techniques. Results: Both extracts were not possessed
anticancer activity to KB‑oral cavity cancer, MCF7‑breast cancer and NCI‑H187‑small
lung cancer cell lines at maximum concentration = 50 µg/mL. In the same way
to anticancer activity assays, both extracts were not inhibited HSV‑1, P. falciparum
K1 strain, M. tuberculosis H37Ra strain and B. cereus at the same concentration.
Conclusions: Ethanol and water extracts of leaves and shooting parts of seablite had
no significant anticancer and antimicrobial activities.