Study The Biologic Effects Of Thymus Kotschyanus With Chlorhexidine 0.2% Mouthmouthwash Solution On Some Mouth And Theet Bacteria In Vitro Condition

Abstract

Thymus kotschyanus is an essential oil with high antimicrobial effects. The present research was aimed to assess the antimicrobial effects of T. kotschyanus essential oil, chlorhexidine 0.2% mouthwash and antibiotic agents against some mouth and theet bacteria in vitro condition. Aerial parts of the T. kotschyanus were prepared and used for essential oil extraction. Chemical components of T. kotschyanus essential oil were identified using the GC-MS. Antimicrobial effects were examined using the disk diffusion and MIC and MBC determination. Totally, 25 chemical compounents were identified in the T. kotschyanus essential oil. Carvacrol (52.01%), Sabinene hydrate (11.58%), 1-8 cineol (7.50%), Para saimene (6.89%) were the most commonly identified chemical compounents in the T. kotschyanus essential oil. In the disk diffusion, the diameter of the growth inhibition zones had ranges between 5.15±0.16 to 14.80±1.09 mm. The highest diameter of the growth inhibition zone was recorded for L. casei treated with T. kotschyanus (10 mg/ml), while the lowest was found for S. salvarius treated with T. kotschyanus (0.16 mg/ml). Statistically significant differences were obatined between some concentration of the T. kotschyanus and chlorhexidine, ampicillin, and metronidazol antibiotic agents (P <0.05). The MIC values had ranges between 2.5 to 5 mg/ml. The MBC values hgd ranges between 5 to 10 mg/ml. The lowest MIC was identified for S. sanguis, S. salvarius, L. reutrei, L. acidophilus and L. casei treated by T. kotschyanus essentialk oil (2.5 mg/ml). The lowest MBC was identified for S. sanguis, S. salvarius, L. reutrei, L. acidophilus and L. casei treated by T. kotschyanus essentialk oil (5 mg/ml). According to the edible route of T. kotschyanus essential oil and its higher antimicrobial effects compatred to antibiotic agents and chlorhexidine mouthwash, formulation of mouthwash based on the T. kotschyanus essential oil is recommended to decrease the risk of oral infections, dental caries, and their subsequent complications.