Tizanidine Intranasal Nanoemulsion In situ Gel: Formulation and In-Vivo Brain Study

Authors

  • Ahmed Y. Fadhel
  • Nawal A. Rajab

Keywords:

Nanoemulsion, Insuito gel, pH, Gelation time, Ethanol.

Abstract

In this work, intranasal nanoemulsion in situ gel (NIG) was developed as new dosage form for tizanidine. An o/w nanoemulsion (NE) was used, which composed from tween 80 as surfactant and ethanol as co-surfactant with ratio 1:2, oleic acid was used as oily phase. The prepared nanoemulsion formula was used to prepare nanoemulsion in situ gel (NIG) using poloxamer 407 as temperature dependent polymer. NIG formulas were evaluated for pH, drug content, sol-gel transition temperature, viscosity, spreadability, gelation time, in vitro drug release and in vivo brain study (using rabbits) in comparison with conventional in situ-gel (IG).
The pH, spreadability, % drug content, sol-gel transition temperature, and gelation capacity for the selected NIG were 6.2, 4.9 cm, 98.5%, 33 oC and ++ respectively. The in-vitro drug release study of the best NIG2 formula showed 100% after 4h.
In-vivo brain studies showed significant increase in Cmax and AUC (P < 0.05) in the cerebrospinal fluid of brain for the selected NIG2 formula in comparison to in situ gel (IG) prepared by the conventional method indicating the contribution of nanoemulsion insuito gel technique for enhancing drug permeability and hence increase in drug reach to cerebrospinal fluid.

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Published

2022-09-16

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Section

Articles

How to Cite

Tizanidine Intranasal Nanoemulsion In situ Gel: Formulation and In-Vivo Brain Study. (2022). Journal of Pharmaceutical Negative Results, 13(2), 582-591. https://pnrjournal.com/index.php/home/article/view/670