Extraction and Purification of Intracellular Beta-Lactamase from Proteus mirabilis

Authors

  • Tuqa Hasan Hadi, Entesar Hussain Ali

Abstract

P. mirabilis was isolated from UTI patient to ensure that the isolate was belonging to P. mirabilis various tests are made such as staining techniques, biochemical assay, morphological and sensitivity test. The gram stain and biochemical test result show rod pink gram negative bacteria and ensure that the isolate was belong to (P. mirabilis). Optimization education for bacterial growth were done by used more than pH and temperature and it was found that the best conditions for the production and increase the number of bacteria at pH 7.7 with bacterial number and Temperature 37°C cell pellet was re-used as a crude enzymatic extract and discard supernatant cell. Purification of Beta-Lactamase enzyme was accomplished by using Ion exchange and gel filtrations chromatographic techniques. The result shows that, gel filtration has optimal specific activity and purification fold at (28.71U/mg), purification fold 2.05 times.

Downloads

Published

2022-11-15

Issue

Section

Articles

How to Cite

Extraction and Purification of Intracellular Beta-Lactamase from Proteus mirabilis. (2022). Journal of Pharmaceutical Negative Results, 13(4), 1288-1294. https://pnrjournal.com/index.php/home/article/view/3448