Dinucleotide signature of fimh gene of Uropathogenic E. coli isolated from public hospitals in Iraq

Abstract

Introduction: Uropathogenic E. coli (UPEC) is a common cause urinary tract infection (UTI). Type-1 fimbriae [FimH] mediate binding to D-mannose-containing structures and thereby enable UPEC to colonize various host tissues.
Methods: A total of the100 urine samples were obtained from patients suffering from UTI, in a period ranging from February to August 2021. Biochemical test for fimH and bioinformatics approached were made on it, to evaluate dinucleotide signature among samples.
Results: A 63 (68.18%) isolates of the (100 isolates), only showed positive for E.coli. when secerned on the fimH gene of positive E.coli samples, the FimH quality was found in 61(96.8%)samples of 63 UPEC strains isolates.
Conclusion: This study showed the relative abundance of nucleotides is a special feature of the genome and is considered a "genomic signature". By dividing the observed frequency of a specific dinucleotide by its expected frequency, the sequence's nucleotide composition was filtered (predicted based on the mononucleotide composition). Genomic signatures or dinucleotide signature (D.S) are unique to the entire genome and are not dependent on coding areas.