Synthesis of Cus Nanoparticle and Characterization, as well as Investigation of their Anticancer Activity Against A Human Breast Cancer Cell Line

Abstract

CuS nanoparticles were prepared in a novel way, and their efficacy as an anti-cancer agent was investigated, as well as their toxicity, using
a new, simple, easy, and low-cost method called chemical co-precipitation, which involved reacting dehydrated metal chloride salts of the
formula MCl2.2H2O with aqueous sodium sulfide of the formula Na2S.H2O under reaction conditions 0.5 M and the best pH 6 at 35°C. CuS
nanoparticles were identified using X-ray diffraction, FTIR spectroscopy, spectroscopy Energy-dispersive X-rays (EDX), scanning electron
microscopy (SEM), and the activity of the prepared CuS nanoparticles was investigated using an MTT test on the Mcf7 cell line. Graphpad
prism8.0 was used to perform statistical analysis on the results. CuS nanoparticles were 14.43 nm. The efficacy of CuS nanoparticles as an
anti-tumor agent was compared with letrozole the efficacy has been demonstrated using different concentrations on the Mcf7 cell line, with
the highest inhibition rate of 87.49 % after 24 hours and 95.67 % after 48 hours of incubation at a concentration of 400 g/ml, and the lowest
inhibition rate of 1.67 % after 24hrs and 6.35 % after 48 hrs at a concentration of 1.67 g/ml. The maximum percentage of inhibition is 84.61
% after 24 hours and 96.68 percent after 48 hours of incubation at 400 g/ml, while a minimum was 9.1 % after 24hrs and 18.24 after 48 hrs
at 25 g/ml.