Estimation of Some Biomarkers among Patients Suffering from Pyelonephritis and Detection of Uropathogenic Escherichia Coli in Hilla City

Abstract

The current study include (75) samples of patients with pyelonephritis who were admitted clinically private for three months
between March and May 2020 for this investigation, Alpha-2-Macroglobulin was estimated in patients suffering from pyelonephritis and positive culture of UPEC by using Alpha-2-Macroglobulin Enzyme-linked Immunosorbent Assay Kit, the results
showed that, there were significant difference between study groups (patient’s positive culture of UPEC and control group). The
mean differences of Alpha-2-Macroglobulin was significant increase and between patient’s positive culture of UPEC compared
with control group. However, Procalcitonin was estimated in patients suffering from pyelonephritis and positive culture of
UPEC, the results showed that, there were significant difference between study groups (patient’s positive culture of UPEC
and control group). The mean differences of Procalcitonin were significant increase and between patient’s positive culture of
UPEC compared with control group. All samples were culture in various aerobic media and the result show out of seventy-five
samples only 62(82.7%) was positive growth while 13(17.3%) no growth observed. 25 (40.33 %) of the positive cultures were
cultured on selective media (EMB) were diagnosed as E. coli, while 37 (59.67 %) isolates were related to other species of
bacteria. Molecular detection of specific uropathogenic E. coli genes were done in all E. coli isolates from urine samples, when
compared to an allelic ladder, the results showed that all 25 (100%) E. coli isolates had positive results for the chuA gene due
to the presence of (221) bp bands. In addition, a molecular investigation of the uidA gene was performed for all 25 isolates
previously identified as E. coli. This gene can be identified using the (PCR) polymerase chain reaction, which is more sensitive
and specific Tanique. When compared to an allelic ladder, the results showed that all 25 (100%) E. coli isolates had positive
results for the uidA gene due to the presence of (259) bp bands.
Aim to study: The study aims to estimation of some biomarkers such as α-macroglobulin and procalcitonin among patients
suffering from pyelonephritis and detection of uropathogenic Escherichia coli in Hilla City.