Physiological and Histopathological Studies on Bisphenol - A Induced Oxidative Stress in Albino Rats: Amelioration by Green and White Tea Extracts

Authors

  • Abdulrahman Aeed Salih Alfahdawi
  • Israa Mahmood Mohammed
  • Othman Aeed Salih Alfahdawi
  • Khabat Anwar Ali

Abstract

Due to highly exposure of BPA which is harmful substance, we investigate its role in induction of oxidative stress, its effect on pancreatic tissue and some physiological parameters in serum of rats, also make a compartive study of antioxidant role for both green and white tea alcoholic exctracts against this harmful substance. In our experiment 20 male albino rats had been used, their body weights were ranged between 180 and 200 grams. Four equal groups of rats were formed, (five animals each). The first group was treated with corn oil alone, while the second group received 500 mg/kg BPA liquified in corn oil, while the third group received BPA (500 mg/kg) and 300 mg/kg extract of green tea together, and the fourth group received BPA plus 300 mg/kg white tea alcoholic extract tea extract. Animal dosing was orally for 60 days. Level of serum MDA significantly inceased in rat group supplied with PBA while treatment with white and green tea alcoholic extract decreased MDA level. Serum superoxidedismutase (SOD) significantly decreased in PBA group while green tea significantly elevated antioxidant level, rats treated with white tea also showed elevation in serum SOD but it was non significant, indicating higher enhancment of green tea for anioxidant production than white tea. Histopathological examination revealded hemorrahge, apoptosis, necrosis and infiltration of inflammatory cells in pancreatic tissue of PBA of rats group, while treatment with green and white tea alcoholic extract nearly recovered histological architectur of pancreatic islets. Histochemical examination indicated depletion of mucopolysacchride by using a stain named alcian blue periodic- acid schiff stain (AB-PAS), protein also depleted when pancreatic tissue stained with bromophenol blue in rats supplied with PBA, while green tea showed better recovery for protein distribution than white tea.

Downloads

Published

2022-10-15

Issue

Section

Articles

How to Cite

Physiological and Histopathological Studies on Bisphenol - A Induced Oxidative Stress in Albino Rats: Amelioration by Green and White Tea Extracts. (2022). Journal of Pharmaceutical Negative Results, 13(4), 545-553. https://pnrjournal.com/index.php/home/article/view/1865