Negative Pharmacokinetics Evaluation of Liquorice and Pomegranate Juices on Potential of Salbutamol in Rats


  • Wael Abu Dayyih


Liquorice, Negative, Pharmacokinetics Parameters, Pomegranate, Preclinical, Salbutamol


Objectives: Beverages or food may alter the pharmacokinetic parameters and pharmacodynamics of drugs-substances’ active
pharmaceutical ingredients which may cause adverse circumstances. Salbutamol is widely indicated to prevent asthma and acute/chronic
obstructive pulmonary disorders. Liquorice and/or Pomegranate are prescribed to support cough and asthma management. Nonetheless, an
interaction thereafter with a Salbutamol pharmacokinetics was not investigated yet. Thus, the aim of this research is to evaluate the effect of
Liquorice and/or Pomegranate on Salbutamol pharmacokinetic-parameters in animals (rats).
Methods and Materials: A Chromatography method of (HPLC) was carried out. Partially validated after a development profitably
conformed to the European Medical Agency’s guidelines to measure Salbutamol in rat serum. Liquorice and/or Pomegranate juices were
given to rats for three days and, an hour sooner than Salbutamol (5.0 mg/kg), it was administered as an oral solution. Blood samples were
collected at scheduled intervals, then handled and investigated by HPLC methodology.
Results: The linearity of the calibration curve was over 98%. The intra-day and the inter-day accuracy values of Salbutamol were (97.93%-
105.18%), and (98.67%-105.91%), respectively. While the intra- and inter-day precision values were equal to or less than 3.178% and
3.653%, respectively. In addition, no significant change was seen in the pharmacokinetic parameters of Salbutamol when administered to
rats that were given either pomegranate or Liquorice juice.
Conclusions: It was established that the given Liquorice or pomegranate juices with the above-mentioned orally administrated Salbutamol
solution, did not result in a significant parameter in the pharmacokinetic interaction







How to Cite

Negative Pharmacokinetics Evaluation of Liquorice and Pomegranate Juices on Potential of Salbutamol in Rats. (2021). Journal of Pharmaceutical Negative Results, 12(1), 48-53.