Triazole‑chalcones: Lack of Antibacterial, Anti‑candida, and Anti‑dengue Virus Activities

Abstract

Background: The need to identify new antimicrobial drugs that present different mechanisms of action is urgent. The variety of biological
properties of chalcones give them a potential therapeutic compounds. Chalcones’ synthetic manipulations are investigated worldwide in search
of more powerful and efficient drugs to several infectious diseases’ treatment. Objective: The purpose of this work was to evaluate the potential
antibacterial, antifungal, and anti‑dengue virus(DENV) of new ten triazole chalcones. Materials and Methods: Triazole chalcones were used
to determine its antibacterial activity against Gram‑positive bacteria (Staphylococcus aureus and Staphylococcus epidermidis), Gram‑negative
bacteria (Klebsiella pneumoniae, Enterobacter cloacae, Acinetobacter baumannii, and Pseudomonas aeruginosa) species, and anti‑Candida
activity (Candida albicans) by the broth microdilution assay. The anti‑DENV activity was evaluated by cell viability quantification after viral
infection by 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide colorimetric assay. Results: Although Triazole chalcones have
shown a low cytotoxicity concentration (with CC50 of 47.21–192.51 µg/mL), these compounds did not exhibit antimicrobial activity against
Gram‑negative and Gram‑positive bacteria, C. albicans neither DENV, even in the highest concentration tested. Triazole chalcones had no activity
against all tested microorganisms, except for Gram‑positive S. epidermidis bacteria when a poor antimicrobial effect was observed (125 µg/
mL). Conclusion: The results suggest that triazole chalcones investigated in this study are not promising antimicrobial agents.