Evaluation of the Activity of Tontelea micrantha Extracts against Bacteria, Candida and Mayaro virus

Abstract

Objectives: This work aimed to evaluate the antibacterial, antifungal, and anti‑Mayaro virus(MAYV) activity of leaf and branch extracts from
Tontelea micrantha. Materials and Methods: T. micrantha extracts were prepared through the partition of the leaf and branch samples in
different solvents. Then, the antibacterial and antifungal activity was assessed against bacterial pathogens and Candida sp. by the determination
of the minimum inhibitory concentration (MIC) by the broth microdilution method. The activity against anti‑MAYV was evaluated through the
quantification of the extract concentration that promoted the protection of 50% of the cells after the viral infection. Results: The extracts of T.
micrantha were inactive (MIC >500 µg/mL) against Gram‑positive, Gram‑negative and Candida species at the highest concentration tested
(500 µg/mL). Anti‑MAYV activity was also not detected, with SI <10, ranging from 1.2 to 3.6. Conclusion: Although it is used in traditional
medicine, Leaf and branch extracts from T. micrantha did not present antimicrobial activity, which could be caused by the antagonistic effect
of the compounds present in the extract.