Formulation And Characterization Of Andrographolide-Loaded Acrylate Polymer - Containing Nanosuspension For The Treatment Of Ocular Infections: In-Vitro

Abstract

Ocular infections are a significant cause of visual impairment worldwide, and there is a need for new and effective treatment options. Andrographolide, a natural compound, has shown promise in treating ocular infections, but its low solubility and poor bioavailability have limited its therapeutic potential. In this study, we aimed to develop and characterize an andrographolide-loaded nanosuspension using an acrylate polymer and evaluate its in-vitro activity against common ocular pathogens.

The nanosuspension was formulated using a quassi emulsification solvent diffusion method, and its physicochemical properties were characterized using Fourier-transform infrared spectroscopy (FTIR), encapsulation efficiency (EE), particle size, zeta potential, surface morphology, and pH determination. The FTIR analysis revealed the compatibility between andrographolide and the acrylate polymer. The nanosuspension demonstrated high EE and had a small particle size with a narrow size distribution. The zeta potential measurements indicated the stability of the nanosuspension, and the surface morphology showed a smooth and spherical structure. The pH determination indicated the nanosuspension was neutral and suitable for ocular use.

In-vitro evaluation of the andrographolide-loaded nanosuspension showed significant antibacterial activity against Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli, with low cytotoxicity against human corneal epithelial cells. The findings of this study suggest that the andrographolide-loaded nanosuspension has the potential to be an effective and safe treatment for ocular infections, overcoming the limitations of current treatments. Further studies are required to investigate its in-vivo activity and safety in animal models and eventually in clinical trials.

In conclusion, the andrographolide-loaded acrylate polymer-containing nanosuspension developed in this study is a promising formulation for the treatment of ocular infections. The comprehensive characterization of the nanosuspension's physicochemical properties adds to the understanding of its formulation, and the in-vitro evaluation highlights its potential for clinical application.