Bioactive Synergistic Efficacy And Modulatory Responses Of Combined Oleic And Linoleic Acids Supplementation In Lipopolysaccharide-Induced Pulmonary Hypertensive

Abstract

Hypertension is one of the risk factors in developing cardiovascular diseases which is the leading cause of mortality worldwide, and diets have been known to play an important role in the prevention and management of hypertension. Previous studies have assessed the anti-hypertensive effects of oleic and linoleic acids by treating experimental animals with oils containing them. However, this study evaluates the bioactive protective efficacy and modulatory responses of combined oleic and linoleic acids supplementation in lipopolysaccharide-induced pulmonary hypertensive in male rats. 42 healthy adult male Wistar rats weighing between 120-150g were procured and used for the study. The animals were distributed into 6 groups (n = 7) as follows; control group, LPS group, (OLE+ LINO + LPS) group, (OLEIC) group, (LINOLEIC) group, and (OLE + LINO) group. Lipopolysaccharide (0.001µg/kg) was administered for five days by intraperitoneal injection, and the blood pressure of the experimental animals was measured using the non-invasive tail-cuff blood pressure monitor. Each rat in the pretreatment group received 10 mg/kg (per os) of oleic and linoleic acids for a duration of three days before LPS administration. The results of this study show that pre-treatment with combined oleic and linoleic acid significantly (p<0.05) reduced angiotensin II, improved reno-protection by significantly (p<0.05) reducing levels of uric acid in the respective tissues assayed and the levels of hepatic injury markers were improved by significant (p<0.05) increase in the levels of ACE and angiotensin II in the group treated with LPS compared to other experimental groups. This study suggests that pre-treatment with combined oleic and linoleic acids have significant effects on blood pressure following lipopolysaccharide administration. Moreover, it reverses significant deleterious effects that lipopolysaccharide poses to various organs and systems in Wistar rats.