Formulation And Characterization Of Bauhinia Racemosa Extract Loaded Nanoemulsion By Box Behnken Design

Abstract

Nanoemulsions are a type of colloidal dispersions in which nano-sized droplets of one liquid are dispersed in another liquid. Bauhinia racemosa, commonly known as the mountain ebony, is a tropical tree which is widely used in traditional Indian medicine in the form of its leaves and barks. In this study, a novel nanoemulsion of Bauhinia racemosa was developed to enhance the efficacy of the active compounds present in the plant. The Box-Behnken design was used to optimize the formulation variables of the nanoemulsion. The raw material was extracted using with ethanol, and then subjected to a nanoemulsification process. The resulting nanoemulsions were characterized for their physical properties such as particle size, droplet size distribution, zeta potential, and viscosity. The formulation F2 was found to be stable with an average droplet size of 148.96nm and a zeta potential of -38.74mV. Furthermore, in vitro release studies of the nanoemulsion revealed a sustained release pattern with an increased bioavailability of the active compounds. The results of this study demonstrate that the Bauhinia racemosa nanoemulsion could be a promising delivery system for the traditional medicine.