The Impact Of Carbon-Nano Tubes On Morphological, Photosynthetic Pigments, Protein Content And Enzyme Activity Of In-Vitro Multiplication Of Cucumber

Authors

  • Mohanad T. Meften, A.B. Abdel-Razik, S. A. Ibrahim , Ahmed S. Mohamed

DOI:

https://doi.org/10.47750/pnr.2023.14.S01.185

Abstract

This experiment was performed on cucumber (Cucumis sativus) in Ain Shams Center of Genetic Engineering and Biotechnology (ACGEB), Fac. of Agric., Ain Shams Univ., Egypt, during the period from 2020 - 2022 to investigate the responses of cucumber seedlings to different concentrations of multi-walled carbon nanotubes "MWCNT" incorporation into the culture medium. Cucumber seeds were planted in hormone – free agar medium. 21 days’ later, stem nots were collected and were divided into 7 groups to be planted in media containing different concentrations of MWCNT (0.5 mg/l, 1 mg/l, 1.5 mg/l, 2 mg/l and 2.5 mg/l MWCNT) in parallel with two other sets, as control, one of them was planted in medium contains 0.5 ml/l of royal water without MWCNT and the other set without either MWCNT or royal water. Results indicated that the applications of different concentrations of MWCNT improved seedling growth. The MWCNT 1.5 and MWCNT 2 mg/l treatments significantly improved the plant length and leaves number respectively comparing to the untreated control. The CNT 2.5 mg/l and CNT 2 mg/l treatments achieved the highest increase in chlorophyll-a chlorophyll-b and total chlorophyll contents. Also the SDS-PAGE total protein showed that the intensity of protein bands increases visibly starting from the concentration 0.1 mg/l moreover, new bands at 100 KD, 75 KD and 20 KD emerged. Peroxidases isozyme analysis showed that peroxidase 1 was produced more intensely at concentrations 1 mg/l, 1.5 mg/l and 2 mg/l of MWCNT while peroxidase 2, 3, 4 and 5 analysis showed contradicting and variable results.

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Published

— Updated on 2023-02-15

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Articles

How to Cite

The Impact Of Carbon-Nano Tubes On Morphological, Photosynthetic Pigments, Protein Content And Enzyme Activity Of In-Vitro Multiplication Of Cucumber. (2023). Journal of Pharmaceutical Negative Results, 1361-1366. https://doi.org/10.47750/pnr.2023.14.S01.185