EVALUATION OF ANTI ATHEROGENIC ACTIVITY OF FLAVONOIDS (3,3',4',5-TETRA HYDROXY-6,8-DIMETHOXY FLAVONE) ISOLATED FROM Cadaba farinosa Forsk IN RAT FED WITH ATHEROGENIC DIET

Abstract

The present investigation to decide the flavonoids isolated from Cadaba farinosa (Linn) has been screened for its antiatherogenic activity. We have isolated flavonoids by column chromatography on the methanolic extract of Cadaba farinosa. Flavonoids 1 from Cadaba farinosa were evaluated at a fixed dose of 15 mg/kg/day. The atherogenic diet (AD) is used to induce hyperlipidemia in wistar albino rats, and the plasma levels of various biochemical markers, including total cholesterol, triglycerides, LDL, VLDL, and HDL cholesterol, as well as invivo antioxidant enzymes like SOD and CAT, are then measured. 24 male Wister rats were distributed into the four groups of six rats each as follows: Standard chow diet was administered to group 1, an atherogenic diet was administered to group 2, Rats in groups 3 and 6 received atorvastatin (1.2 mg/kg B.wt.) and AD with flavonoids from Cadaba farinosa (15 mg/kg B.wt.). After an overnight fast, all rats were slaughtered at the end of three weeks via cervical dislocation. When compared to groups of rats treated with AD, the flavonoids (CF1) from Cadaba farinosa-administered rats had significantly lower body weight, plasma and tissue total cholesterol, TG, phospholipids, plasma Low density lipoprotein, and Very Low density lipoprotein, as well as higher plasma levels of HDL, SOD, and CAT. We came to the conclusion that the flavonoids (CF1) from Cadaba farinosa are a significant hypolipidemic agent with preventative and therapeutic activity against hyperlipidemia after considering the findings.