LC/MS characterization of secondary metabolites of Sphagneticola trilobata J. F Pruski Leaves

Abstract

Spectroscopic and chromatographic methods were derived to identify the secondary metabolites from Sphagneticola trilobata, an essential medicinal plant. Invitro DPPH radical scavenging activity of different fractions was carried out by DPPH assay. The LC/MS analysis was performed so as to determine characteristic active fractions. Several phytoconstituents such as Rhamnetin 3- laminaribiosideMethyl 7-epi-12- hydroxyjasmonate glucoside8-Epiiridodial glucoside tetraacetateMethyl 7-epi-12- hydroxyjasmonate glucosideCamellianin A Gibberellin A110, 3-Hydroxychavicol 1- [rhamnosyl-(1->6)- glucoside]4'-O-methyl-(-)- epicatechin-3'-O-betaglucuronide6-Ketoestriol Rhamnetin 3-laminaribioside 3,4-Dicaffeoyl-1,5-quinolactone Methyl 7-epi-12- hydroxyjasmonate glucosideIridodial glucoside tetraacetate Eugenol O-[a-L-Arabinofuranosyl-(1- >6)-b-D-glucopyranoside]4'-O-methyl-(-)-epicatechin-3'- O-beta-glucuronide2',7-Dihydroxy-4'-methoxy-8- prenylflavan 2',7-diglucosidexi-3-Hydroxy-5-phenylpentanoic acid O-beta-D-Glucopyranoside were identified  for first time, invitro  and invivo  studies are to be reported in further studies. The characterization of S.trilobata proved to  be helpful for the potential use of this plant in novel medicinal products.