A COMPARATIVE STUDY OF ANTI-INFLAMMATORY EFFECT OF THIOCOLCHICOSIDE, DICLOFENAC AND THEIR COMBINATION IN WISTAR RATS USING CARRAGEENAN INDUCED PAW EDEMA METHOD

Abstract

AIM: To study anti-inflammatory effects of thiocolchicoside in wistar rats in comparison with the standard drugs.

OBJECTIVES:

1. To study anti-inflammatory effect of thiocolchicoside, diclofenac and combination of thiocolchicoside & diclofenac in wistar rats.
2. To compare anti-inflammatory effect of thiocolchicoside, diclofenac and combination of thiocolchicoside & diclofenac in wistar rats.

MATERIAL AND METHODS: Anti-inflammatory activity was evaluated by using carrageenan induced paw edema method on plethysmograph.  Wistar rats divided into 5 groups (n =6) Group 1 (control group) which was given 0.9% Normal Saline. Group 2 (test group) was treated with Thiocolchicoside 2mg/kg (ip). Group 3 (test group) was treated with Thiocolchicoside 4mg/kg (ip). Group 4 is standard treatment group where Diclofenac 10mg/kg (ip) was given. Group 5 was treated with Thiocolchicoside 2mg/kg + Diclofenac 10mg/kg. Inhibition of paw edema was recorded at 1 hr, 2 hr, 3 hr, 4 hr time intervals after treatment.

RESULTS: Significant anti-inflammatory activity was observed in group 3 (Thiocolchicoside 4mg/kg) and Group 4 (Diclofenac 10mg/kg), and except at 1^st hr interval in Group 5 (Thiocolchicoside 2mg/kg + Diclofenac 10mg/kg) Statistically significant anti-inflammatory activity was observed (p<0.05) in test drug treatment group 3 (Thiocolchicoside 4mg/kg) and group 4 (Diclofenac 10mg/kg) and except at 1^st hr interval in Group 5 (combination of Thiocolchicoside 2mg/kg + Diclofenac 10mg/kg) when compared to group 1 (Normal Saline 0.1ml/100gm). Thiocolchicoside 2mg/kg did not add to anti-inflammatory activity of Diclofenac 10mg/kg.

CONCLUSION: Thiocolchicoside showed time dependent and dose dependent rise in anti-inflammatory activity.