Formulation Development And Characterization Of In Situ Gel Containing Bimatoprost For The Treatment Of Glaucoma

Abstract

The In Situ gelling system has existed as a promising drug delivery system in the ophthalmic domain in recent times. The present research focuses on the development and evaluation of In Situ gelling system for targeting Glaucoma to enhance ocular residence time, and increasing the bioavailability. In Situ gelling formulations were developed by selecting temperature sensitive polymer like Pluronic F-127 and viscosifying agents like HPMC K4M in different concentrations. The prepared In Situ Gels were characterized for its appearance, pH, clarity, gelation studies, drug content, gelling capacity, gelation temperature, rheological studies and in vitro drug release studies. The optimized formulation underwent ex vivo permeation studies, microbial test, in vivo ocular irritancy and stability studies.  After the characterization, BT-4 gave the most promising profile of release of drug. This formulation was clear in appearance and sol to gel transition was achieved quickly on approaching the environment of body temperature. The formulation was not irritant on ocular surface and further it was found stable when undergone for a period of three months stability testing. Bimatoprost in situ gelling system could be a promising drug delivery system in treatment of glaucoma by reducing the intra ocular pressure in controlled manner with enhanced bioavailability.