Molecular Characterization of Yeasts Isolated from the Urinary Tract of Pregnant Women in Kirkuk City

Abstract

The current study aimed to the molecular diagnosis of yeasts isolated during the study. This study was conducted in the Graduate Studies Laboratory / College of Science - Kirkuk University, and included the isolation and diagnosis of yeasts that cause urinary tract infections in pregnant women, and they were diagnosed by routine laboratory methods, and the diagnosis of yeasts was confirmed using polymerase chain reaction (PCR) technology. 200 urine samples were collected from pregnant women with urinary tract infection who attended the maternity, gynecological and children’s hospital in Kirkuk governorate and their ages ranged between 17-43 years, in the period between November 2021 and until February 2022. The results showed that the sequencing was performed for the selected isolates and entered into the NCBI BLAST program to identify the similarity between them and the results in the global gene bank after obtaining the nucleotide sequence of the DNA package of the local isolates and comparing it with the sequences of the same region for some global and local Candida spp isolates registered in NCBI BLAST, where Candida albicans showed a match between 99.80 to 99.39 percent with the results in the gene bank, and Candida glabrata showed a match with the gene bank with a percentage of 99.85% to 98.82%, and for the type Pichia kudriavzevii (Candida krusei) it was 100%.