The Rapid Pcr Detection Of Potentially Pathogenic Bacteria In The Drinking Water Of Different Sources From Bhopal District Of Madhya Pradesh

Abstract

One of the essentials for human survival is access to clean, sufficient drinking water (WHO, 2015). But the major problems in the world, especially in developing nations, is the lack of availability of clean drinking water and challenges with its quality, Hence the present study were planned to study the presence of bacteria in the drinking water samples of Bhopal, Madhya Pradesh.The 200water samples were collected from household point-of-use (POU) in a pre-autoclaved vial. Nutrient Agar is a nutrient medium has usedfor the to cultivate of microorganisms. The NAM plates were incubated in incubator at 37 ⁰C overnight for the desired bacterial growth. The each randomly selected appeared bacterial colony was taken in the individual autoclaved PCR tube, preformed the Colony PCR for the detection of thePsedomonas sp., Aeromonas sp., Escherichia coli and Salmonela sp. using bacteria specific primers for PCR.The Colony PCR accurately confirmed the presence of pathogenic bacterial species with moderate prevalence of Psedomonassp.,Aeromonas sp. and Salmonella sp. but Escherichia coli sp. showed high prevalence in drinking water samples from Bhopal districts of Madhya Pradesh, the presence of all pathogenic bacterial species could be one of the major threats to the health of the human population of the study area.