Identification Of Bioactive Phytochemicals By GC-MS Profiling, Phytochemical Screening, And TLC Using Methanolic Extract Of Carissa Carandas Linn Leaf

Abstract

Background Carissa carandas (Apocynaceae) is a thorny evergreen shrub known as Karonda. It has small berry-shaped fruits, used as an additive in many pickles or spices in northern India.

Objective The objective was to identify the phytochemical constituents, gas chromatography-mass spectrometry (GC-MS), and TLC screening by using a methanolic extract from the leaves of Carissa carandas.

Methods and materials Leaves of C. carandas were collected, shade dried, pulverized, and extracted successively with methanol by the Soxhlet technique. The crude extracts were later exposed to phytochemical screening for secondary metabolites, thin-layer chromatography, and GC-MS analysis.

Result The phytochemical screening revealed the presence of alkaloids, saponin, tannin, flavonoid, phenol, steroid, carbohydrate, glycosides, and terpenoids. TLC analysis was performed, and the Rf value was calculated. The GC-MS analysis determined the presence of 6 main phytochemical compounds in the methanolic extract of Carissa carandas leaf, showing that there were different types of high and low molecular weight compounds.

Conclusion  The results of the present study pave the invention of herbal medicines for several ailments by using the Carissa carandas plant, which may lead to the development of novel drugs.