The Use Of Nutrient Media Of Different Composition To Increase The Number Of Potato Microtubers

Authors

  • Etzaeva K.T. , Oves E. V.

DOI:

https://doi.org/10.47750/pnr.2023.14.S01.47

Abstract

The article presents the results of studies on the use of combined compositions of MS culture media with sucrose content from 2 to 8% using test tubes with a diameter of 16 and 22 mm and plastic containers 18x18cm. The aim of the research was to study the elements of microtuber cultivation technology that increase the yield of the standard fraction (more than 8 mm). The highest yield of microtubers was noted when using containers with a combined composition of nutrient media (MS agar medium with 2% sucrose added after formation of 4-5 internodes of MS liquid medium with 8% sucrose). In such cases, when using test-tube technology, the studied potato varieties formed from 1.3 to 1.4 microtubers with a standard fraction yield of 0.9 to 1.1 pieces, or 69 - 78%. For microplants in containers, the addition of MS liquid medium with 8% sucrose at the second stage of plant development increased the yield of microtubers. Their number increased to 1.9 - 2.2 pieces. at the exit of the standard fraction 1.4 -1.7 pcs. Thus, the cultivation of microtubers in containers contributed to an increase in the quantitative yield of microtubers compared to the test-tube culture by 1.5–1.9 times in the Gulliver variety, 1.8–2.2 times in the Sadon variety, and 1.7–2.1 times in the variety Kumach. At the same time, the yield of the standard fraction as a result of the use of containers increased by 1.3–1.9 times compared to the test-tube culture. The introduction of microtuber cultivation technology into the laboratory process facilitates year-round in vitro cultivation of material and is used as an adjunct to a clonal micropropagation program.

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Published

2023-01-01 — Updated on 2023-01-01

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Articles

How to Cite

The Use Of Nutrient Media Of Different Composition To Increase The Number Of Potato Microtubers. (2023). Journal of Pharmaceutical Negative Results, 423-427. https://doi.org/10.47750/pnr.2023.14.S01.47