Diagnostic Implications Of Urine 8-Hydroxy-2-Deoxyguanosine (8-Ohdg) As A Sensitive Biomarker For Early Prediction Of Diabetic Kidney Disease Among Adolescents With Type 1 Diabetes Mellitus

Authors

  • Shimaa Atef , Basant Ahmed Abd El-Alim , Rasha Essam Eldin Galal , Laila Ahmed Rashed , Mona Mamdouh Hassan

DOI:

https://doi.org/10.47750/pnr.2023.14.S01.20

Abstract

Background: The most prevalent and severe microvascular complication in individuals who have type 1 diabetes (T1DM) is diabetic kidney disease (DKD), and its severity is assessed by the degree of albuminuria, but it lacks the sensitivity and specificity required to identify DKD in an early stage; necessitating the use of other biomarkers. One of these biomarkers is 8-Hydroxy-2-Deoxyguanosine (8-OHdG) in urine. Aim: To evaluate urine 8-OHdG's sensitivity and specificity for earlier identification of DKD. Methods: Ninety-one study participants were recruited as 64 adolescents with T1DM and 27 healthy control subjects. According to the results of their urine albumin/creatinine ratio (UACR), T1DM patients were classified into three groups: normoalbuminuric group, intermittent microalbuminuric group, and persistent microalbuminuric group. Blood pressure and anthropometric measurements were performed. Laboratory tests were requested within 3 months of T1DM patient recruitment. All participants' urine samples were taken at the time of their enrollment, and ELISA kits were used to check for urine 8-OHdG. Results: Mean glycated hemoglobin (HbA1c) was above the target level (≥7%) in 96.9% of participants with T1DM. T1DM patients' mean urine 8-OHdG was considerably greater in comparison to the control group, additionally those with persistent or intermittent microalbuminuria also had higher mean levels than those with normoalbuminuria. There was highly statistically considerable difference between the three groups of T1DM regarding urine 8-OHdG. We found that urine 8-OHdG is unable to discriminate between persistent and intermittent microalbuminuria, but can fairly discriminate between normoalbuminuria and persistent microalbuminuria, as well as normoalbuminuria and intermittent microalbuminuria. Conclusion: Nine T1DM patients with normoalbuminuria had urine 8-OHdG levels higher than the cutoff limit, so longitudinal follow up of those patients is recommended to make sure that they will develop microalbuminuria later on, and at that time we can prove that urine 8-OHdG can be used for earlier detection of DKD.

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Published

2023-01-01 — Updated on 2023-01-01

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How to Cite

Diagnostic Implications Of Urine 8-Hydroxy-2-Deoxyguanosine (8-Ohdg) As A Sensitive Biomarker For Early Prediction Of Diabetic Kidney Disease Among Adolescents With Type 1 Diabetes Mellitus. (2023). Journal of Pharmaceutical Negative Results, 185-195. https://doi.org/10.47750/pnr.2023.14.S01.20