The evaluation of Paclitaxel effects on the Caco-2 cell line of colon cancer patients

Abstract

Background: Colon cancer is considered a malignant neoplasm. Paclitaxel is anti-carcinogenic chemotherapy that stimulates the apoptosis of cancer cells.
Objective: This study aims to know the effect of paclitaxel on the level of Reactive oxygen species (ROS) on Caco-2 of colon cancer cells by the High-content screening technique.
Methods: Normal and cancerous human colon cells (Caco-2cell lines) were cultured, then treated with paclitaxel at two different quantitative doses (100 μg/ml) and (200 μg/ml) for 1-7 days. The examination was carried out at the University of Malaya Kuala Lumpur, College of Medicine, specifically at the Natural Products Research Center and the Department of Drug Discovery in Pharmacology in Malaysia.
Results: The high-concentration paclitaxel doses (P => 0.0001) were more effective than the low-concentration paclitaxel doses (P = 0.0043) when compared with the healthy person as the high dose had a significant effect in inhibiting the growth of cancer cells on days 7 by stimulating ROS secretion in the cells. The epithelium of the colon (CaCO-2). High-content screening used to detect oxidative stress.
Conclusion: Higher doses of paclitaxel are more effective over time to inhibit the growth and spread of colon cancerous cells and reduce the effects on normal colonic epithelial cell growth. The increased level of ROS in the Caco-2 cell line appears to be associated with the increased dose concentration of paclitaxel.