Spectrophotometric Determination of Prednisolone Drug Using Cloud Point Extraction and pharmaceutical application

Abstract

In this paper, describes a separation and Preconcentration method for the determination of Prednisolone. extract Prednisolone (Pred) in the form of an ionic bonding complex, and the complexing agent FeCl3.6H2O was used in this compound UV-VIS wavelength spectrum to confirm its structural formula on its greatest wave 390.5 nm Optimum conditions for extraction and spectroscopic determination of Pred were found: Pred concentration and reagent (100 μg.ml-1) (1×10-3M) respectively, Triton-114 V/V (10%) . and heating (10min), centrifugal speed of 4000 r/min , the linear concentrations range that obeyed Beer's is (2-18 μg.ml-1) and value correlation coefficient R2 = 0.995 , limit of detection LOD (0.0293 μg ∙ ml-1), limit of quantification LOQ (0.0888 μg ∙ ml-1), and mole ratio were studied in order to optimize the reaction conditions. The mole ratio for the composition of product is (1:3). molar absorption coefficient (21798 l mol-1. cm) , Sandel sensitivity (0.0222 μg.cm-2).