Bacteriological and molecular study of the effect of magnetic field on Methicillin Resistance Staphylococcus aureus (MRSA)

Authors

  • Qanat Mahmood Atiyea
  • Rafea Z. alsugmiany
  • Fatima Mustafa Al-najar
  • Arshad M Hamad

DOI:

https://doi.org/10.47750/pnr.2022.13.S01.19

Keywords:

Staphylococcus aureus, magnetic field, antibiotic, polymerase chain reaction.

Abstract

A group of staphylococcal bacteria strains were exposed to the magnetic field and the characteristics of these bacteria were studied and diagnosed through phenotypic and biochemical characteristics. In addition to studying the sensitivity of these isolates to antibiotics, which included ( AK 30, Lev 5, Tm 5, VA 5, Met 10, CEC 5, SAM 20, GM 10, OX 5, NIT 100, CIP 5) and noted the differences between these antibiotics in terms of its effect on the bacterial isolates under study, where the isolates resistant to methicillin ( Methicillin Resistance Staphylococcus aureus ) were identified (3 thee isolates B1,B2,B3) and then exposed to the magnetic field with one strength and one dimension, but at different times (30 sec, 45 sec, 60 sec), where it was observed that the number of bacterial cells decreased in lower concentrations, but by the influence of the magnetic field. Molecular markers: Most of treatments were distinguished with unique bands and absent bands the all mutant bands (28). unique bands (21) and absent bands (7), As for the B3 obtained mutant bands which reached (11) bands of which (8) unique bands and (3) Absent bands. As for the B2 obtained which reached (7) bands of which (6) unique bands and (1) Absent band. As for the B1 obtained which reached (6) bands of which (4) unique bands and (2) Absent bands. The molecular differences between the strains were determined from the molecular side at the stage before and after exposure to the magnetic field through random primers used in the polymerase chain reaction technique. There is a difference in the results between the three bacterial because of the difference in the genome of them due to different sources. The magnetic field creates mutations in the genome of bacterial . and the mutation increases with the period of exposure of the plant to the magnetic field. The efficiency of the RAPD markers was high in detecting the producing mutation in the genome of bacterial.

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Published

2022-09-15

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Articles

How to Cite

Bacteriological and molecular study of the effect of magnetic field on Methicillin Resistance Staphylococcus aureus (MRSA). (2022). Journal of Pharmaceutical Negative Results, 147-154. https://doi.org/10.47750/pnr.2022.13.S01.19