Expression of rhlR gene in Pseudomonas aeruginosa affected by Lactobacillus spp

Abstract

P. aeruginosa is an opportunistic pathogen causes severe infections due to distinct virulence factors such as pyocyanin. This pigment confers the microbe severity during the infections. This study aimed to evaluate metabolites from Lactobacillus on pyocyanin production. Seven isolates of P. aeruginosa were screened for pyocyanin. All the isolates were produced pyocyanin in rate ranged between 0.47 to 0.82 µg/ml.The isolates were AHL producers with absorbance at 630 nm ranged between 2.26 to 4.88. The culture filtrate from Lactobacillus spp. showed inhibition activity against pyocyanin production. The clinical Lactobacillus spp. was characterised by their higher ability to inhibit pyocyanin production compared to the environmental isolate. There was a clear variation in folding change values in expression of rhlRgene after treatment with Lactobacillus filtrate. Treatment with CF of Lac9 was up-regulated 1.89-Fold the transcription of rhlR gene. The results of this study suggest that there is a clear interaction between P. aeruginosa and Lactobacillus regarding the pyocyanin production.