Association between the Genetic Variation of LIF R Gene with Its Gene Expression and Implantation Outcomes of IVF-Iraqi Arab Females

Abstract

Background:  Infertility causes an important social and psychological stressor amongst couples everywhere, particularly among   Iraqi women.  It   is a couple's failure to conceive   following   one year   at least   of unprotected intercourse. Only 15% of in vitro grown embryos are implanted successfully. LIF R   genes variants  are a new crucial bio-indicator for predicting the success of IVF implantation amongst  infertility females. LIF R gene   is participates in the blastocyst   implantation into   the endometrium.   Also,   the   LIF R   gene  act as an anti-inflammatory function, when tissue inflammation. Therefore, LIF R gene expression increased during implantation.

 Study Aims: The present study aims to   determine effect gene expression and genetic variants of the leukemia inhibitory factor receptor (LIF R) gene   in the implantation outcomes  in female Iraqi Arab infertility patients.

Materials and  Methods: Samples were collected from Kamal Al-Samarrai hospital due to  the period from December 2019 to November 2020. The samples were split  based on IVF implantation outcomes.

 Results:.  At first time: the current study recorded  a new SNP 5557, (C>G) of  the LIF R gene among the  success implantation group only.   While, rs1012350134 SNP 65661, (A>G)   was affected (P<0.001) through modify GG as a risk factor in failed IVF implantation 72.7% than 33.3% in successful implantation.    AG frequency has statistically important  9.1% for failure  comparison 58.3% in success implantation.  Reduced gene expression of  LIF R genes is caused by polymorphism in the LIF R genes. 

 Conclusion: The genetic variance can be screening method for endometrial receptivity, to IVF -implantation improving, or susceptibility risk of   failure implantation