Preparation of Monolithic Chromatographic Column for Propranolol Hydrochloride Determination

Abstract

Pharmacuticalcal compounds can be separated, identified, and quantified using high-pressure liquid chromatography (HPLC) in analytical chemistry. A monolithic column of glycidyl methacrylate-ethylene dimethcrylate-co-acryl amide was prepared by in-situ co-polymerization inside; a borosilicate tube as a mould (60mm in length) with 1.5mm and 3.0 mm (i.d. and o.d.) for LC separation. Monomers (glycidyl methacrylate and acrylamide) and a cross-linker (ethylene dimethacrylate) were dissolved in a porogenic solvent of 1-propanol, and hexanol is a suitable solvent for monomers and cross-linker. Nevertheless, it is a poor solvent for the prepared monolith. The polymer was formed after 3 min using UV- polymerization at 365 nm. Glycidyl methacrylate's epoxy groups were changed to diol groups by pumping 0.2 M HCl through the column. FT-IR, BET and FE-SEM were utilized to investigate and prove the monolith formation. The monolith was used to determine propranolol hydrochloride in medications.