EVALUATION OF RICE BRAN OIL FROM RICEBERRY RICE (ORYZA SATIVA L.) AS ACTIVE INGREDIENT IN SKINCARE APPLICATION

Abstract

Rice bran oil (RBO) from riceberry rice is process from rice bran and contain health promoting benefits due to their biological activities. This study was aimed to determine phenolic, flavonoid and tannin contents of RBO and evaluate biological activities including in vitro antioxidant, anti-inflammation, anti-microbials, anti-tyrosinase, anti-elastase, wound-healing promotion and cytotoxicity of RBO against human skin fibroblast cells. Cold (mechanical) pressing was commercialized RBO extraction. Total phenolic content (TPC), total flavonoid content (TFC) and total tannin content (TTC) of RBO were determined by colorimetric absorbance measurements of reaction mixture. Antioxidant property of RBO was evaluated by DPPH, NO radical scavenging and lipid peroxidation inhibition. In vitro anti-inflammation of RBO was evaluated by inhibition of NO production of LPS-induced macrophages. Anti-tyrosinase and anti-elastase of RBO were also evaluated according colorimetric methods. RBO cytotoxicity and wound healing was evaluated against human skin fibroblast cells by sulforhodamine B staining with survival cells and closed gap of cell cluster scratching, respectively. Anti-microbials of RBO was determined against four skin pathogens by disc diffusion method. TPC, TFC and TTC contained were 1.41±0.11 mg of GAE/g, 6.61±0.11 mg of QE/g and 47.89±0.75 mg of TE/g, respectively. RBO was scavenged DPPH and NO radicals (IC50 = 2.42 ±0.11 and 69.9±5.9 mg/ml). RBO (1.0 mg/ml) was exhibited in vitro anti-inflammation and almost similar to positive control. RBO was slightly activated cell proliferation at 0.0001 to 0.1 mg/ml and had cytotoxicity at 1.0 mg/ml. However, there was lack of anti-tyrosinase, anti-elastase anti-microbial and wound healing activities.